fig , confocal fluorescence images of bt cells stained with mitotracker red after exposure for lohrs to dna green complexed with cdqasomes left column circular mlspdna conjugate, right column linearized mlspdna conjugate top row a and b red channel, middle row c and d green channel, bottom row e and f corresponding overlaid images figure shows confocal fluorescence micrographs of cells incubated with mlspdna conjugates, which were vectorized with vesicles made from tests for lipitor side effects the cyclohexyl derivative of dequalinium cdqasomes for the cell exposures tests for lipitor side effects imaged in the left column panels �, � and e tests for lipitor side effects the nonrestricted, ie circular form of pdna was used, while for the experiments pictured in the right column panels b, d tests for lipitor side effects and f, the plasmid dna was linearized before dqaplex formation tests for lipitor side effects the characteristic red mitochondrial staining pattern panels a and b shows the functional viability of the imaged cells and the intracellular green fluorescence panels � and d demonstrates efficient cell internalization of the fluorescein labeled dna the green and red fluorescence channels tests for lipitor side effects were then overlaid to produce the composite image seen in tests for lipitor side effects panels e and f, where the regions of true colocalization tests for lipitor side effects of red and green fluorescence were pseudocolored in white for better tests for lipitor side effects visualization strikingly, in the overlaid images, there is hardly any tests for lipitor side effects green fluorescence detectable nearly all areas of green fluorescence in tests for lipitor side effects panels � and d appeared as white areas in panels e tests for lipitor side effects and f, strongly suggesting that almost the entire dna has tests for lipitor side effects been delivered not only towards mitochondria, but also into the organelle however, whether all or at least a portion of the pdna has actually entered the mitochondrial matrix, ie has crossed tests for lipitor side effects both mitochondrial membranes, and therefore would potentially be accessible to tests for lipitor side effects the mitochondrial transcription machinery, remains yet to be determined dqasomes as carriers of proapoptotic drugs dysregulation of the apoptotic machinery is generally accepted as an almost universal component of the transformation process of normal cells into cancer cells and a large body of experimental data demonstrates that mitochondria play a key role in the complex apoptotic mechanism consequently, any therapeutic strategy aimed tests for lipitor side effects at specifically triggering apoptosis in cancer cells is believed to tests for lipitor side effects have potential therapeutic effect, several clinically approved drugs such as vp etoposide, arsenite and vinorelbine, as well as an increasing number of experimental anticancer drugs reviewed by constantini et al, such tests for lipitor side effects as betulinic acid, lonidamine, ceramide and cd have been found to tests for lipitor side effects act directly on mitochondria, resulting in triggering apoptosis in order tests for lipitor side effects to maximize the therapeutic potential of such anticancer drugs, which are known to act at or inside mitochondria, the use of tests for lipitor side effects dqasomes as a mitochondriaspecific drug delivery system has been proposed hypothetically, dqasomebased anticancer chemotherapy entails features which would make it tests for lipitor side effects putatively superior to conventional chemotherapeutic approaches on the cellular, as well tests for lipitor side effects as the subcellular level firstly, the delivery of drugs known tests for lipitor side effects to act directly on mitochondria may trigger apoptosis in circumstances in which conventional drugs fail to act, because endogenous, upstream of mitochondria apoptosis induction pathways are disrupted secondly, transporting the cytotoxic cipro litigation tests for lipitor side effects drug to its intracellular target could overcome multidrug resistance by hiding the drug inside the delivery system until it becomes selectively released at the particular intracellular site of action, ie mitochondria thirdly, many carcinoma cells, including human breast adenocarcinoma derived cells, tests for lipitor side effects have an elevated plasma membrane potential relative to their normal tests for lipitor side effects parent cell lines in addition to the higher mitochondrial membrane potential, they could provide the basis for a doubletargeting effect of tests for lipitor side effects dqasomes, ie on the cellular level normal cells vs carcinoma cells, and on the subcellular level mitochondria versus nucleus first data tests for lipitor side effects involving the encapsulation of anticancer drugs into dqasomes have been published most recently in this study, paclitaxel was chosen as a model compound paclitaxel is known as a potent antitubulin agent used in the treatment of malignancies its therapeutic potential, however, tests for lipitor side effects is limited due to a very narrow span between the tests for lipitor side effects maximal tolerated dose and intolerable toxic levels in addition, its poor aqueous solubility requires the formulation of emulsions containing cremophor el�, tests for lipitor side effects an oil of considerable toxicity by itself recently, it has tests for lipitor side effects been demonstrated that clinically relevant concentrations of paclitaxel target mitochondria directly and trigger apoptosis by inducing cytochrome � release in a tests for lipitor side effects permeability transition pore ptpdependent manner this mechanism of action is known from the other proapoptotic, directly on mitochondria acting agents a tests for lipitor side effects hour delay between the treatment with paclitaxel or with other ptp inducers, and the release of cytochrome � in cellfree tests for lipitor side effects systems, compared with intact cells, has been explained by the existence of several drug targets inside the cell, making only a subset of the drug available for mitochondria consequently, paclitaxel was tests for lipitor side effects considered a prime candidate to benefit from a mitochondriaspecific drug delivery system such as dqasomes it was demonstrated that paclitaxel can tests for lipitor side effects be incorporated into dqasomes at a stoichiometric molar ratio of paclitaxel to dequalinium considering the known spherical character of dqasomes, the results of an electron microscopic em analysis of dequasomal incorporated tests for lipitor side effects paclitaxel, however, seem rather surprising the transmission em image fig , tests for lipitor side effects left panel and the cryoem image fig of an identical sample show a remarkable conformity worm or rodlike structures approximately nm in length, the size of which could also be confirmed by the size distribution analysis shown in fig , right panel the molecular structureof this wormlike complex remains to be determined nevertheless fig left panel transmission electron microscopic image uranyl acetate staining of dqasomal incorporated paclitaxel mol taxolmol dequalinium right panel size distribution analysis of identical preparation shown in left panel the formation of wormlike micelles as described for selfassembling amphiphilic block copolymers appears possible � s � i in a preliminary study, tests for lipitor side effects paclitaxelloaded dqasomes were tested for their ability to inhibit the growth of human colon cancer cells in nude mice for controls with free paclitaxel, the drug was suspended in dmso at mm, stored at �c and immediately diluted in warm medium before tests for lipitor side effects use in all controls, the respective dose of free paclitaxel and empty dqasomes was adjusted according to the dose of paclitaxel and dequalinium given in the paclitaxelloaded dqasome sample due to tests for lipitor side effects the lack of any inhibitory effect on tumor growth, the dose was tripled after weeks figure shows that at concentrations where free paclitaxel and r hepes buffer v free paclitaxel empty dqasomes ?